Recombinant:Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness.
Article Snippet: Particle (Locus ID 2841) OriGene Technologies Cat#TL312655V .. Biological samples Human serum From subject cohort - ClinicalTrials.gov ID-NCT02719899 N/A Chemicals, peptides, and recombinant proteins Arachidonoyl Glycine Cayman Chemical Company Cat#90051; CAS: 179113-91-8 PSB-KD107 Cayman Chemical Company Cat#31393; CAS: 955121-65-0 PSB-CB5 Tocris Cat6372; CAS: 1627710-30-8 Human IL-2 Recombinant protein Peprotech Cat#200-02B Critical commercial assays Human CD4+T cell Isolation Kit Miltenyi Biotec Cat#130-091-155 CyQUANT LDH Cytotoxicity Assay Invitrogen Cat#C20300 CyQUANT Cell Proliferation Assay Invitrogen Cat#C7026 Human IFNg Duoset ELISA Kit R&D Systems Cat#DY285B Human IL-4 Duoset ELISA Kit R&D Systems Cat#DY204 Human IL-17 Duoset ELISA Kit R&D Systems Cat#DY317 Deposited data Human Metabolome Database (HMDB, Version 4.0) Wishart et al.52 https://hmdb.ca/ RNAseq of PBMCs This paper; Han et al.13 Table S3; GEO: GSE165149 RNAseq of subcutaneous adipose tissue biopsies Rey et al.35 GEO: GSE166047 (Continued on next page) iScience 26, 106578, May 19, 2023 13 .. Serum metabolomics data This paper; Mendeley Data: https://data.mendeley.com/ datasets/zvzvd5f5cb/1 Table S2; https://doi.org/10.17632/zvzvd5f5cb.1
Cell Isolation:Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness.
Article Snippet: Particle (Locus ID 2841) OriGene Technologies Cat#TL312655V .. Biological samples Human serum From subject cohort - ClinicalTrials.gov ID-NCT02719899 N/A Chemicals, peptides, and recombinant proteins Arachidonoyl Glycine Cayman Chemical Company Cat#90051; CAS: 179113-91-8 PSB-KD107 Cayman Chemical Company Cat#31393; CAS: 955121-65-0 PSB-CB5 Tocris Cat6372; CAS: 1627710-30-8 Human IL-2 Recombinant protein Peprotech Cat#200-02B Critical commercial assays Human CD4+T cell Isolation Kit Miltenyi Biotec Cat#130-091-155 CyQUANT LDH Cytotoxicity Assay Invitrogen Cat#C20300 CyQUANT Cell Proliferation Assay Invitrogen Cat#C7026 Human IFNg Duoset ELISA Kit R&D Systems Cat#DY285B Human IL-4 Duoset ELISA Kit R&D Systems Cat#DY204 Human IL-17 Duoset ELISA Kit R&D Systems Cat#DY317 Deposited data Human Metabolome Database (HMDB, Version 4.0) Wishart et al.52 https://hmdb.ca/ RNAseq of PBMCs This paper; Han et al.13 Table S3; GEO: GSE165149 RNAseq of subcutaneous adipose tissue biopsies Rey et al.35 GEO: GSE166047 (Continued on next page) iScience 26, 106578, May 19, 2023 13 .. Serum metabolomics data This paper; Mendeley Data: https://data.mendeley.com/ datasets/zvzvd5f5cb/1 Table S2; https://doi.org/10.17632/zvzvd5f5cb.1
CyQUANT Assay:Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness.
Article Snippet: Particle (Locus ID 2841) OriGene Technologies Cat#TL312655V .. Biological samples Human serum From subject cohort - ClinicalTrials.gov ID-NCT02719899 N/A Chemicals, peptides, and recombinant proteins Arachidonoyl Glycine Cayman Chemical Company Cat#90051; CAS: 179113-91-8 PSB-KD107 Cayman Chemical Company Cat#31393; CAS: 955121-65-0 PSB-CB5 Tocris Cat6372; CAS: 1627710-30-8 Human IL-2 Recombinant protein Peprotech Cat#200-02B Critical commercial assays Human CD4+T cell Isolation Kit Miltenyi Biotec Cat#130-091-155 CyQUANT LDH Cytotoxicity Assay Invitrogen Cat#C20300 CyQUANT Cell Proliferation Assay Invitrogen Cat#C7026 Human IFNg Duoset ELISA Kit R&D Systems Cat#DY285B Human IL-4 Duoset ELISA Kit R&D Systems Cat#DY204 Human IL-17 Duoset ELISA Kit R&D Systems Cat#DY317 Deposited data Human Metabolome Database (HMDB, Version 4.0) Wishart et al.52 https://hmdb.ca/ RNAseq of PBMCs This paper; Han et al.13 Table S3; GEO: GSE165149 RNAseq of subcutaneous adipose tissue biopsies Rey et al.35 GEO: GSE166047 (Continued on next page) iScience 26, 106578, May 19, 2023 13 .. Serum metabolomics data This paper; Mendeley Data: https://data.mendeley.com/ datasets/zvzvd5f5cb/1 Table S2; https://doi.org/10.17632/zvzvd5f5cb.1
LDH Cytotoxicity Assay:Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness.
Article Snippet: Particle (Locus ID 2841) OriGene Technologies Cat#TL312655V .. Biological samples Human serum From subject cohort - ClinicalTrials.gov ID-NCT02719899 N/A Chemicals, peptides, and recombinant proteins Arachidonoyl Glycine Cayman Chemical Company Cat#90051; CAS: 179113-91-8 PSB-KD107 Cayman Chemical Company Cat#31393; CAS: 955121-65-0 PSB-CB5 Tocris Cat6372; CAS: 1627710-30-8 Human IL-2 Recombinant protein Peprotech Cat#200-02B Critical commercial assays Human CD4+T cell Isolation Kit Miltenyi Biotec Cat#130-091-155 CyQUANT LDH Cytotoxicity Assay Invitrogen Cat#C20300 CyQUANT Cell Proliferation Assay Invitrogen Cat#C7026 Human IFNg Duoset ELISA Kit R&D Systems Cat#DY285B Human IL-4 Duoset ELISA Kit R&D Systems Cat#DY204 Human IL-17 Duoset ELISA Kit R&D Systems Cat#DY317 Deposited data Human Metabolome Database (HMDB, Version 4.0) Wishart et al.52 https://hmdb.ca/ RNAseq of PBMCs This paper; Han et al.13 Table S3; GEO: GSE165149 RNAseq of subcutaneous adipose tissue biopsies Rey et al.35 GEO: GSE166047 (Continued on next page) iScience 26, 106578, May 19, 2023 13 .. Serum metabolomics data This paper; Mendeley Data: https://data.mendeley.com/ datasets/zvzvd5f5cb/1 Table S2; https://doi.org/10.17632/zvzvd5f5cb.1
Proliferation Assay:Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness.
Article Snippet: Particle (Locus ID 2841) OriGene Technologies Cat#TL312655V .. Biological samples Human serum From subject cohort - ClinicalTrials.gov ID-NCT02719899 N/A Chemicals, peptides, and recombinant proteins Arachidonoyl Glycine Cayman Chemical Company Cat#90051; CAS: 179113-91-8 PSB-KD107 Cayman Chemical Company Cat#31393; CAS: 955121-65-0 PSB-CB5 Tocris Cat6372; CAS: 1627710-30-8 Human IL-2 Recombinant protein Peprotech Cat#200-02B Critical commercial assays Human CD4+T cell Isolation Kit Miltenyi Biotec Cat#130-091-155 CyQUANT LDH Cytotoxicity Assay Invitrogen Cat#C20300 CyQUANT Cell Proliferation Assay Invitrogen Cat#C7026 Human IFNg Duoset ELISA Kit R&D Systems Cat#DY285B Human IL-4 Duoset ELISA Kit R&D Systems Cat#DY204 Human IL-17 Duoset ELISA Kit R&D Systems Cat#DY317 Deposited data Human Metabolome Database (HMDB, Version 4.0) Wishart et al.52 https://hmdb.ca/ RNAseq of PBMCs This paper; Han et al.13 Table S3; GEO: GSE165149 RNAseq of subcutaneous adipose tissue biopsies Rey et al.35 GEO: GSE166047 (Continued on next page) iScience 26, 106578, May 19, 2023 13 .. Serum metabolomics data This paper; Mendeley Data: https://data.mendeley.com/ datasets/zvzvd5f5cb/1 Table S2; https://doi.org/10.17632/zvzvd5f5cb.1
Enzyme-linked Immunosorbent Assay:Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness.
Article Snippet: Particle (Locus ID 2841) OriGene Technologies Cat#TL312655V .. Biological samples Human serum From subject cohort - ClinicalTrials.gov ID-NCT02719899 N/A Chemicals, peptides, and recombinant proteins Arachidonoyl Glycine Cayman Chemical Company Cat#90051; CAS: 179113-91-8 PSB-KD107 Cayman Chemical Company Cat#31393; CAS: 955121-65-0 PSB-CB5 Tocris Cat6372; CAS: 1627710-30-8 Human IL-2 Recombinant protein Peprotech Cat#200-02B Critical commercial assays Human CD4+T cell Isolation Kit Miltenyi Biotec Cat#130-091-155 CyQUANT LDH Cytotoxicity Assay Invitrogen Cat#C20300 CyQUANT Cell Proliferation Assay Invitrogen Cat#C7026 Human IFNg Duoset ELISA Kit R&D Systems Cat#DY285B Human IL-4 Duoset ELISA Kit R&D Systems Cat#DY204 Human IL-17 Duoset ELISA Kit R&D Systems Cat#DY317 Deposited data Human Metabolome Database (HMDB, Version 4.0) Wishart et al.52 https://hmdb.ca/ RNAseq of PBMCs This paper; Han et al.13 Table S3; GEO: GSE165149 RNAseq of subcutaneous adipose tissue biopsies Rey et al.35 GEO: GSE166047 (Continued on next page) iScience 26, 106578, May 19, 2023 13 .. Serum metabolomics data This paper; Mendeley Data: https://data.mendeley.com/ datasets/zvzvd5f5cb/1 Table S2; https://doi.org/10.17632/zvzvd5f5cb.1
RNA sequencing:Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness.
Article Snippet: Particle (Locus ID 2841) OriGene Technologies Cat#TL312655V .. Biological samples Human serum From subject cohort - ClinicalTrials.gov ID-NCT02719899 N/A Chemicals, peptides, and recombinant proteins Arachidonoyl Glycine Cayman Chemical Company Cat#90051; CAS: 179113-91-8 PSB-KD107 Cayman Chemical Company Cat#31393; CAS: 955121-65-0 PSB-CB5 Tocris Cat6372; CAS: 1627710-30-8 Human IL-2 Recombinant protein Peprotech Cat#200-02B Critical commercial assays Human CD4+T cell Isolation Kit Miltenyi Biotec Cat#130-091-155 CyQUANT LDH Cytotoxicity Assay Invitrogen Cat#C20300 CyQUANT Cell Proliferation Assay Invitrogen Cat#C7026 Human IFNg Duoset ELISA Kit R&D Systems Cat#DY285B Human IL-4 Duoset ELISA Kit R&D Systems Cat#DY204 Human IL-17 Duoset ELISA Kit R&D Systems Cat#DY317 Deposited data Human Metabolome Database (HMDB, Version 4.0) Wishart et al.52 https://hmdb.ca/ RNAseq of PBMCs This paper; Han et al.13 Table S3; GEO: GSE165149 RNAseq of subcutaneous adipose tissue biopsies Rey et al.35 GEO: GSE166047 (Continued on next page) iScience 26, 106578, May 19, 2023 13 .. Serum metabolomics data This paper; Mendeley Data: https://data.mendeley.com/ datasets/zvzvd5f5cb/1 Table S2; https://doi.org/10.17632/zvzvd5f5cb.1
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